Highlight Photo
Sequencing by Ligation Variation with Endonuclease V, Deoxyinosine, and SAWTooth The Sequencing Analysis Workbench Tool
Sequencing by Ligation
Cyclic SBL involves using Endonuclease V to recognize an incorporated deoxyinosine site and clip the DNA. This results in a fragment of the query primer attached to the anchor primer, allowing subsequent ligations to sequence further out.
Credits: Antoine Ho – University of New Mexico
